The PIGW Antibody (PAC058240) is a polyclonal antibody designed for research involving the PIGW protein, an enzyme involved in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it a valuable tool for studying the role of PIGW in various cellular processes.The PIGW protein is essential for GPI anchor biosynthesis, a process that is crucial for anchoring proteins to the cell membrane and regulating cell signaling. Dysfunction of PIGW has been linked to various diseases, including neurological disorders and cancer, making it an important target for research in these areas.
This antibody is validated for use in Western blot applications, enabling the detection and analysis of PIGW protein levels in different cell types. Its high specificity and sensitivity make it ideal for studies in cell biology, biochemistry, and disease mechanisms related to GPI anchor biosynthesis. By understanding the function of PIGW, researchers can gain insights into the underlying mechanisms of disease and potentially identify new therapeutic targets for treatment.
Antibody Name:
PIGW Antibody (PACO58240)
Antibody SKU:
PACO58240
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:5000
Species Reactivity:
Human, Rat
Immunogen:
Recombinant Human Phosphatidylinositol-glycan biosynthesis class W protein (392-448AA)
Western Blot. Positive WB detected in: Rat kidney tissue. All lanes: PIGW antibody at 3.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57 kDa. Observed band size: 57 kDa.
Background:
Required for the transport of GPI-anchored proteins to the plasma membrane. Probable acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor. Acetylation during GPI-anchor biosynthesis is not essential for the subsequent mannosylation and is usually removed soon after the attachment of GPIs to proteins (By similarity).
Synonyms:
Phosphatidylinositol-glycan biosynthesis class W protein (PIG-W) (EC 2.3), PIGW
UniProt Protein Function:
Required for the transport of GPI-anchored proteins to the plasma membrane (PubMed:24367057). Probable acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor. Acetylation during GPI-anchor biosynthesis is not essential for the subsequent mannosylation and is usually removed soon after the attachment of GPIs to proteins ().
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is an inositol acyltransferase that acylates the inositol ring of phosphatidylinositol. This occurs in the endoplasmic reticulum and is a step in the biosynthesis of glycosylphosphatidylinositol (GPI), which anchors many cell surface proteins to the membrane. Defects in this gene are a cause of the age-dependent epileptic encephalopathy West syndrome as well as a syndrome exhibiting hyperphosphatasia and cognitive disability (HPMRS5). [provided by RefSeq, Jul 2017]
