The PIGV Polyclonal Antibody (PAC058492) is a crucial tool for researchers studying PIGV, an essential enzyme involved in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to PIGV protein, this antibody enables accurate detection and analysis in various cell types, making it an invaluable asset for studies in molecular biology and cellular signaling pathways.PIGV plays a vital role in the synthesis of GPI anchors, which are crucial for anchoring proteins to the cell membrane and facilitating cellular communication.
Dysregulation of PIGV activity has been implicated in various diseases, including genetic disorders and cancer. By investigating the function of PIGV, researchers can gain insight into the mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention.Overall, the PIGV Polyclonal Antibody (PAC058492) offers researchers a reliable tool for studying the role of PIGV in cellular processes and disease pathogenesis. Its high specificity and sensitivity make it ideal for a wide range of research applications, making it a valuable addition to any laboratory studying molecular mechanisms and disease pathways related to GPI anchor biosynthesis.
Western Blot. Positive WB detected in: A549 whole cell lysate. All lanes: PIGV antibody at 4.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56 kDa. Observed band size: 56 kDa.
Immunofluorescence staining of Hela cells with PACO58492 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO58492 diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Alpha-1,6-mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the second mannose to the glycosylphosphatidylinositol during GPI precursor assembly.
Synonyms:
GPI mannosyltransferase 2 (EC 2.4.1) (GPI mannosyltransferase II) (GPI-MT-II) (Phosphatidylinositol-glycan biosynthesis class V protein) (PIG-V), PIGV
UniProt Protein Function:
PIGV: Alpha-1,6-mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the second mannose to the glycosylphosphatidylinositol during GPI precursor assembly. Defects in PIGV are the cause of hyperphosphatasia with mental retardation type 1 (HPMRS1). It is a syndrome characterized by elevated serum alkaline phosphatase, severe mental retardation, seizures, hypotonia, facial dysmorphism, and hypoplastic terminal phalanges. Belongs to the PIGV family.Protein type: EC 2.4.1.-; Membrane protein, multi-pass; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Membrane protein, integral; Transferase; Endoplasmic reticulumChromosomal Location of Human Ortholog: 1p36.11Cellular Component: endoplasmic reticulum membrane; mannosyltransferase complexMolecular Function: glycolipid mannosyltransferase activity; mannosyltransferase activityBiological Process: GPI anchor biosynthetic process; preassembly of GPI anchor in ER membraneDisease: Hyperphosphatasia With Mental Retardation Syndrome 1
UniProt Protein Details:
NCBI Summary:
This gene encodes a mannosyltransferase enzyme involved in the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is a complex glycolipid that functions as a membrane anchor for many proteins and plays a role in multiple cellular processes including protein sorting and signal transduction. The encoded protein is localized to the endoplasmic reticulum and transfers the second mannose to the GPI backbone. Mutations in this gene are associated with hyperphosphatasia mental retardation syndrome. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Feb 2011]
