The PIGM Polyclonal Antibody (CAB17811) is a valuable tool for researchers studying the PIGM protein, which is involved in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.PIGM plays a critical role in anchoring proteins to the cell membrane, and mutations in the PIGM gene have been associated with glycosylphosphatidylinositol biosynthesis defect 7, a rare genetic disorder.
By targeting the PIGM protein, researchers can gain insight into GPI anchor biosynthesis and its implications for cellular function and disease.This antibody enables the detection and analysis of PIGM in a variety of cell types, making it a valuable tool for studies in biochemistry, cell biology, and genetics. Understanding the role of PIGM in GPI anchor biosynthesis is essential for unraveling the complexities of this critical cellular process and its relevance to human health.
Product Name:
PIGM Rabbit Polyclonal Antibody
SKU:
CAB17811
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 35-96 of human PIGM (NP_660150.1).
This gene encodes a transmembrane protein that is located in the endoplasmic reticulum and is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI)-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes a mannosyltransferase, GPI-MT-I, that transfers the first mannose to GPI on the lumenal side of the endoplasmic reticulum.
Purification Method:
Affinity purification
Gene ID:
93183
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PIGM Rabbit pAb (CAB17811) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 120s.
