The PIGH Antibody (PAC035450) is a highly specific and sensitive tool for studying the PIGH protein, which plays a key role in the glycosylphosphatidylinositol (GPI) anchor biosynthesis pathway. This polyclonal antibody, developed using rabbits as the host species, has been rigorously validated for use in Western blot applications and shows strong reactivity with human samples.PIGH is essential for the proper attachment of GPI anchors to proteins on the cell surface, which is crucial for various cellular functions including signal transduction, cell adhesion, and immune response. Dysregulation of the GPI anchor biosynthesis pathway, including mutations in PIGH, has been linked to a range of diseases such as paroxysmal nocturnal hemoglobinuria (PNH) and certain neurological disorders.
By using the PAC035450 antibody, researchers can accurately detect and quantify PIGH levels in different cell types and tissues, allowing for a deeper understanding of the protein's functions in health and disease. This antibody is a valuable tool for investigations in molecular biology, cell biology, and clinical research, with potential applications in the development of novel diagnostic and therapeutic strategies.
Antibody Name:
PIGH Antibody (PACO35450)
Antibody SKU:
PACO35450
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:1000-1:5000
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant Human Phosphatidylinositol N-acetylglucosaminyltransferase subunit H protein (1-188AA)
Western blot. All lanes: PIGH antibody at 4µg/ml + Mouse kidney tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 22 kDa. Observed band size: 22 kDa.
Background:
Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.
Synonyms:
Phosphatidylinositol N-acetylglucosaminyltransferase subunit H (EC 2.4.1.198) (Phosphatidylinositol-glycan biosynthesis class H protein) (PIG-H), PIGH
UniProt Protein Function:
PIGH: Part of the complex catalyzing the transfer of N- acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis. Belongs to the PIGH family.Protein type: Transferase; Endoplasmic reticulum; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; EC 2.4.1.198Chromosomal Location of Human Ortholog: 14q24.1Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complexMolecular Function: catalytic activityBiological Process: GPI anchor biosynthetic process; preassembly of GPI anchor in ER membrane; protein modification process
UniProt Protein Details:
NCBI Summary:
This gene encodes an endoplasmic reticulum associated protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. The protein encoded by this gene is a subunit of the GPI N-acetylglucosaminyl (GlcNAc) transferase that transfers GlcNAc to phosphatidylinositol (PI) on the cytoplasmic side of the endoplasmic reticulum. [provided by RefSeq, Jul 2008]
