The PIF1 Antibody (PAC018213) is a highly specific and sensitive tool for researchers studying the PIF1 protein, which is known for its role in DNA replication and repair processes. This polyclonal antibody, derived from rabbits, is optimized for detection in human samples and is suitable for Western blot applications. By binding to the PIF1 protein, this antibody allows for easy detection and analysis in a variety of cell types, making it an ideal choice for investigations in molecular biology and cancer research.PIF1 is a key player in maintaining genomic stability and is involved in various aspects of DNA metabolism, including telomere maintenance and replication stress response.
Dysregulation of PIF1 has been linked to DNA damage and genomic instability, highlighting its importance in understanding the mechanisms underlying diseases such as cancer and genetic disorders. By studying the function of PIF1, researchers can gain insights into potential therapeutic targets for these conditions, paving the way for the development of novel treatments.
Antibody Name:
PIF1 Antibody (PACO18213)
Antibody SKU:
PACO18213
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:1000-1:5000, WB:1:500-1:2000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide of human PIF1
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: 293T cells, Hela cells, Primary antibody: PACO18213(PIF1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute.
Background:
PIF1 is a 5-prime-to-3-prime DNA helicase that negatively regulates telomerase (see TERT; MIM 187270), a reverse transcriptase that maintains telomere length. Tightly cell cycle regulated and expressed in late S/G2 phase.
Synonyms:
ATP-dependent DNA helicase PIF1
UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:
This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013]
