Pichia HCP ELISA Kit (HDES0124)
- SKU:
- HDES0124
- Size:
- 96T
- Detection Method:
- Sandwich ELISA
- Target:
- Pichia HCP
- Detection Wavelength:
- 450/630nm
Description
| Product Name: | Pichia HCP ELISA Kit |
| SKU: | HDES0124 |
| Size: | 96T |
| Target: | Pichia HCP |
| Detection Method: | Sandwich ELISA |
| Result Type: | Quantitative: Sensitivity 98.8%, Specificity 99.6% |
| Sample Type: | Antibodies, Recombinant Proteins, Recombinant Vaccines Etal |
| Assay Time: | 3 h |
| Detection Wavelength: | 450/630nm |
| Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Pichia HCP. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Pichia HCP and Streptavidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Pichia HCP, biotinylated detection antibody and streptavidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Pichia HCP. You can calculate the concentration of Pichia HCP in the samples by comparing the OD of the samples to the standard curve.
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