The PIAS1 Polyclonal Antibody (CAB1007) is a valuable tool for researchers studying PIAS1, a protein implicated in regulating gene expression and cell signaling pathways. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the PIAS1 protein, this antibody enables researchers to detect and analyze PIAS1 expression in various cell types, making it ideal for investigations in the fields of molecular biology and cancer research.PIAS1, also known as Protein Inhibitor of Activated STAT1, plays a crucial role in controlling gene transcription and protein modification processes, ultimately impacting cellular function and response to external stimuli.
Understanding the functions and regulation of PIAS1 is essential for unraveling its involvement in diseases such as cancer, neurodegenerative disorders, and inflammatory conditions. By targeting PIAS1 with this specific antibody, researchers can gain valuable insights into its role in these pathological processes, potentially leading to the development of targeted therapeutic strategies.
Product Name:
PIAS1 Rabbit Polyclonal Antibody
SKU:
CAB1007
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human PIAS1 (NP_057250.1).
This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
8554
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PIAS1 antibody (CAB1007) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
