The PI4KA Polyclonal Antibody (CAB19329) is a valuable tool for researchers studying PI4KA, a key enzyme involved in cellular signaling pathways. The antibody, generated in rabbits, exhibits high specificity and sensitivity towards PI4KA protein in human samples, making it suitable for use in Western blotting applications. By binding to PI4KA, this antibody enables accurate detection and quantification of the enzyme in various cell types, facilitating investigations in cell biology and signal transduction research.
PI4KA is a phosphatidylinositol kinase that plays a critical role in the synthesis of phosphatidylinositol 4-phosphate (PI4P), a lipid molecule essential for membrane trafficking and cellular signaling. Dysregulation of PI4KA has been linked to various diseases, including cancer and neurodegenerative disorders, highlighting its importance as a therapeutic target. Research on PI4KA function and regulation is essential for developing novel treatment strategies that target these diseases at the molecular level.
Product Name:
PI4KA Rabbit Polyclonal Antibody
SKU:
CAB19329
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1780-1860 of human PI4KA (P42356).
Sequence:
PEAI VLDI DYKS GTPM QSAA KAPY LAKF KVKR CGVS ELEK EGLR CRSD SEDE CSTQ EADG QKIS WQAA IFKV GDDC RQDM L
This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by this gene is a type III enzyme that is not inhibited by adenosine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
5297
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PI4KA Rabbit pAb (CAB19329) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
