The PI3 Kinase p85 alpha/beta Monoclonal Antibody is a valuable tool for researchers studying the PI3 Kinase pathway and its role in cell signaling and regulation. This antibody, monoclonal in nature, specifically targets the p85 alpha/beta subunits of PI3 Kinase, which are crucial for its activation and function.The PI3 Kinase pathway is involved in a variety of cellular processes, including cell growth, proliferation, survival, and metabolism. Dysregulation of this pathway has been implicated in various diseases, including cancer, diabetes, and inflammatory disorders. The ability to specifically target the p85 alpha/beta subunits with this antibody allows for detailed analysis of their expression levels, localization, and post-translational modifications in different cell types and experimental conditions.
The PI3 Kinase p85 alpha/beta Monoclonal Antibody is suitable for use in Western blot, immunoprecipitation, and immunofluorescence applications. Its high specificity and sensitivity make it an excellent choice for researchers seeking to elucidate the complex mechanisms underlying PI3 Kinase signaling and its impact on cellular physiology and disease pathology.
Product Name:
PI3 Kinase p85 alpha/beta Monoclonal Antibody
SKU:
CAB22487
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human PI3 Kinase p85 alpha/beta (NP_852664.1).
cell-cell junction, cis-Golgi network, cytoplasm, cytosol, nucleus, perinuclear region of cytoplasm, plasma membrane
Calculated MW:
42-54kDa/81-84kDa
Observed MW:
85kDa
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
Purification Method:
Affinity purification
Gene ID:
5295/5296
Clone Number:
ARC57210
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PI3 Kinase p85 alpha/beta Rabbit mAb (CAB22487) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
