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PI3 Kinase p85 alpha/beta Monoclonal Antibody (CAB22487)

SKU:
CAB22487
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €529
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:PI3 Kinase p85 alpha/beta Monoclonal Antibody
SKU:CAB22487
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400-500 of human PI3 Kinase p85 alpha/beta (NP_852664.1).
Sequence:SVVE LINH YRNE SLAQ YNPK LDVK LLYP VSKY QQDQ VVKE DNIE AVGK KLHE YNTQ FQEK SREY DRLY EEYT RTSQ EIQM KRTA IEAF NETI KIFE EQCQ T
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500-1:1000
Synonyms:PIK3R1/PIK3R2; PI3 Kinase p85 alpha/beta
Positive Sample:Jurkat,NIH/3T3,Mouse brain
Conjugate:Unconjugated
Cellular Localization:cell-cell junction, cis-Golgi network, cytoplasm, cytosol, nucleus, perinuclear region of cytoplasm, plasma membrane
Calculated MW:42-54kDa/81-84kDa
Observed MW:85kDa

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Purification Method:Affinity purification
Gene ID:5295/5296
Clone Number:ARC57210
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using PI3 Kinase p85 alpha/beta Rabbit mAb (CAB22487) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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