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PI3 Kinase p85 alpha/beta/gamma Monoclonal Antibody (CAB22996)

SKU:
CAB22996
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
IF
$167 - $635
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:PI3 Kinase p85 alpha/beta/gamma Monoclonal Antibody
SKU:CAB22996
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-500 of human PI3 Kinase p85 alpha/beta (NP_852664.1).
Sequence:SVVE LINH YRNE SLAQ YNPK LDVK LLYP VSKY QQDQ VVKE DNIE AVGK KLHE YNTQ FQEK SREY DRLY EEYT RTSQ EIQM KRTA IEAF NETI KIFE EQCQ T
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:2000 - 1:10000 IF/ICC,1:50 - 1:200
Synonyms:PIK3R1/PIK3R2/PIK3R3
Positive Sample:Mouse brain
Conjugate:Unconjugated
Cellular Localization:Golgi network, Cytoplasm, Cytosol, Nucleus, Perinuclear region of cytoplasm, Plasma membrane
Calculated MW:42-54kDa/81-84kDa
Observed MW:85kDa

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Purification Method:Affinity purification
Gene ID:5295/5296/8503
Clone Number:ARC57209
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using PI3KP85α/P55γ/P85β antibody (CAB22996) at 1:9000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.

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