The PI3 Kinase p85 Alpha/Beta/Gamma Monoclonal Antibody is a valuable tool for researchers studying the PI3 Kinase signaling pathway. This antibody specifically recognizes the p85 subunits of PI3 Kinase, which play a crucial role in regulating cell growth, proliferation, and survival.Raised against the PI3 Kinase p85 alpha, beta, and gamma isoforms, this monoclonal antibody is highly specific and sensitive for detecting these proteins in various cell types and tissues. Validated for use in Western blotting applications, this antibody enables researchers to study the expression and activation of the PI3 Kinase p85 subunits in both normal and diseased cells.The PI3 Kinase signaling pathway is known to be dysregulated in a variety of diseases, including cancer, diabetes, and cardiovascular disorders.
By using this antibody to study the PI3 Kinase p85 subunits, researchers can gain insight into the mechanisms underlying these diseases and potentially identify new therapeutic targets for intervention.Overall, the PI3 Kinase p85 Alpha/Beta/Gamma Monoclonal Antibody is a valuable tool for researchers interested in understanding the role of PI3 Kinase signaling in health and disease. Its high specificity and sensitivity make it an essential reagent for studies investigating the molecular mechanisms of cell growth, proliferation, and survival.
Golgi network, Cytoplasm, Cytosol, Nucleus, Perinuclear region of cytoplasm, Plasma membrane
Calculated MW:
42-54kDa/81-84kDa
Observed MW:
85kDa
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
Purification Method:
Affinity purification
Gene ID:
5295/5296/8503
Clone Number:
ARC57209
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PI3KP85α/P55γ/P85β antibody (CAB22996) at 1:9000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
