PHPT1 Antibody (CAB13590)
- SKU:
- CAB13590
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Anti-PHPT1 Antibody (CAB13590)
The PHPT1 Polyclonal Antibody (CAB13590) is a valuable tool for researchers studying PHPT1, a protein involved in maintaining cellular phosphate homeostasis. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.PHPT1 is a phosphohistidine phosphatase that plays a crucial role in regulating phosphate levels within cells, making it a key player in various cellular processes such as energy metabolism and signal transduction. Dysregulation of PHPT1 has been implicated in diseases such as cancer, osteoporosis, and metabolic disorders, highlighting its importance in human health.
By utilizing the PHPT1 Polyclonal Antibody, researchers can accurately detect and analyze PHPT1 protein levels in different cell types, providing valuable insights into its function and potential therapeutic implications. This antibody is a valuable tool for studies in biochemistry, cell biology, and disease research, offering a deeper understanding of PHPT1's role in maintaining cellular homeostasis.
| Antibody Name: | Anti-PHPT1 Antibody |
| Antibody SKU: | CAB13590 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human PHPT1 (NP_054891.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | MCF7, A-549, THP-1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human PHPT1 (NP_054891.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAVA DLAL IPDV DIDS DGVF KYVL IRVH SAPR SGAP AAES KEIV RGYK WAEY HADI YDKV SGDM QKQG CDCE CLGG GRIS HQSQ DKKI HVYG YSMA YGPA QHAI STEK IKAK YPDY EVTW ANDG Y |
| Gene ID: | 29085 |
| Uniprot: | Q9NRX4 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 13kDa |
| Observed MW: | 13kDa |
| Synonyms: | PHPT1, CGI-202, HEL-S-132P, HSPC141, PHP, PHP14 |
| Background: | This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | PHPT1: a phosphohistidine phosphatase. Present preferentially in heart and skeletal muscle. Insensitive to inhibition by okadaic acid and EDTA. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - fructose and mannose; Phosphatase; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.-; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - thiamine Chromosomal Location of Human Ortholog: 9q34.3 Cellular Component: cytosol Molecular Function:phosphohistidine phosphatase activity; calcium channel inhibitor activity; phosphoprotein phosphatase activity Biological Process: negative regulation of lyase activity; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation |
| NCBI Summary: | This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] |
| UniProt Code: | Q9NRX4 |
| NCBI GenInfo Identifier: | 25008934 |
| NCBI Gene ID: | 29085 |
| NCBI Accession: | Q9NRX4.1 |
| UniProt Secondary Accession: | Q9NRX4,Q9H0Y3, B1AMX0, B1AMX1, |
| UniProt Related Accession: | Q9NRX4 |
| Molecular Weight: | 125 |
| NCBI Full Name: | 14 kDa phosphohistidine phosphatase |
| NCBI Synonym Full Names: | phosphohistidine phosphatase 1 |
| NCBI Official Symbol: | PHPT1Â Â |
| NCBI Official Synonym Symbols: | PHP14; CGI-202; HSPC141; HEL-S-132PÂ Â |
| NCBI Protein Information: | 14 kDa phosphohistidine phosphatase; protein janus-A homolog; sex-regulated protein janus-a; epididymis secretory sperm binding protein Li 132P |
| UniProt Protein Name: | 14 kDa phosphohistidine phosphatase |
| UniProt Synonym Protein Names: | Phosphohistidine phosphatase 1; Protein janus-A homolog |
| Protein Family: | 14 kDa phosphohistidine phosphatase |
| UniProt Gene Name: | PHPT1Â Â |
| UniProt Entry Name: | PHP14_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PHPT1 antibody (CAB13590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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