The Phox2B Polyclonal Antibody is a vital tool for researchers studying the Phox2B protein, a key player in the development of the autonomic nervous system. This antibody, produced in rabbits, exhibits high specificity and sensitivity to human samples, making it ideal for Western blot applications. By targeting the Phox2B protein, this antibody allows for precise detection and analysis in various cell types, making it invaluable for investigations in neurobiology and developmental biology.Phox2B, a transcription factor essential for the proper formation of neurons in the autonomic nervous system, has been linked to disorders such as congenital central hypoventilation syndrome (CCHS).
Research into the function and regulation of Phox2B is crucial for understanding the mechanisms underlying these disorders and potentially uncovering new therapeutic avenues. By utilizing the Phox2B Polyclonal Antibody, researchers can further elucidate the role of Phox2B in neurological development and disease, paving the way for innovative advancements in the field.
Product Name:
PHOX2B Polyclonal Antibody
SKU:
CAB21990
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 260-314 of human PHOX2B (NP_003915.2).
The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. Expansion of a 20 amino acid polyalanine tract in this protein by 5-13 aa has been associated with congenital central hypoventilation syndrome.
Purification Method:
Affinity purification
Gene ID:
8929
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Neuro-2a cells using [KD Validated]PHOX2B Rabbit pAb(CAB21990) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime:10s.
