The Phox2b Polyclonal Antibody (CAB9685) is a valuable tool for researchers studying Phox2b, a transcription factor essential for the development of the autonomic nervous system. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to Phox2b protein, this antibody allows for reliable detection and analysis in a variety of cell types, making it particularly useful for studies in neurobiology and developmental biology.Phox2b is known for its role in the development of the autonomic nervous system, specifically in the differentiation of neurons that control breathing, heart rate, and other vital functions.
Dysregulation of Phox2b has been implicated in various neurological disorders, making it a key target for research into conditions such as congenital central hypoventilation syndrome and neuroblastoma. Understanding the functions and mechanisms of Phox2b is essential for uncovering new insights into these disorders and developing potential therapeutic interventions.
Product Name:
PHOX2B Rabbit Polyclonal Antibody
SKU:
CAB9685
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human PHOX2B (NP_003915.2).
The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. Expansion of a 20 amino acid polyalanine tract in this protein by 5-13 aa has been associated with congenital central hypoventilation syndrome.
Purification Method:
Affinity purification
Gene ID:
8929
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PHOX2B antibody (CAB9685) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
