The Phospholipase A2 Polyclonal Antibody (CAB5478) is a valuable tool for researchers studying phospholipase A2, an enzyme involved in the hydrolysis of phospholipids to produce free fatty acids and lysophospholipids. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications.Phospholipase A2 plays a crucial role in various cellular processes, including inflammation, cell signaling, and membrane remodeling. Dysregulation of phospholipase A2 is implicated in various diseases, such as cardiovascular disorders, neurodegenerative diseases, and inflammatory conditions.
By targeting phospholipase A2 with this antibody, researchers can gain insights into its function and potential therapeutic applications in these pathological conditions.With its ability to detect phospholipase A2 in different cell types and tissues, the Phospholipase A2 Polyclonal Antibody is a valuable tool for studies in lipid metabolism, inflammation, and disease pathology. Its high specificity and reliable performance make it an essential reagent for researchers investigating the role of phospholipase A2 in health and disease.
Product Name:
PLA2G1B Rabbit Polyclonal Antibody
SKU:
CAB5478
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 23-148 of human PLA2G1B (NP_000919.1).
This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response.
Purification Method:
Affinity purification
Gene ID:
5319
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PLA2G1B Rabbit pAb (CAB5478) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
