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Phosphofructokinase（PFK）Activity Assay Kit

SKU:
MAES0262
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Serum, Plasma, Tissue And Cell Sample
Sensitivity:
0.27 U/L
Range:
0.27-32.29 U/L
Assay Time:
15 min
€529
Frequently bought together:

Description

Product Name:Phosphofructokinase（PFK）Activity Assay Kit
Product Code:MAES0262
Sample Type:Serum, Plasma, Tissue And Cell Sample
Assay Time:15 min
Assay Type:Enzyme Activity
Synonyms:PFK
Detection Instrument:Microplate reader (330-350 nm, optimum wavelength: 340 nm)
Detection Method:Colorimetric method
Sensitivity:0.27 U/L
Detection Range:0.27-32.29 U/L
Inter-assay CV:0.08
Intra-assay CV:0.03

Phosphofructokinase(also known as 6-phosphofructokinase; PFK) is a class of kinases that act on fructose-6-phosphate. Phosphofructokinase catalyzes fructose-6-phosphate and ATP to produce fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+. The activity of PFK can be reflected by the determination of NADH decline rate at 340 nm.

Storage:-20°C
Valid Period:12 months
Other Reagents Required:Normal saline (0.9% NaCl)
Other Instruments Required:Centrifuge

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