The Phospho-YAP1 (S127) Polyclonal Antibody (CABP0489) is a valuable tool for researchers studying the Hippo signaling pathway and its role in cell proliferation and apoptosis. This antibody specifically targets phosphorylated YAP1 at serine 127, a key regulatory site that controls YAP1 activity and localization within the cell.YAP1 is a transcriptional co-activator that plays a crucial role in promoting cell growth and survival. Phosphorylation of YAP1 at serine 127 is known to inhibit its nuclear localization and transcriptional activity, leading to its cytoplasmic retention and degradation. Understanding the phosphorylation status of YAP1 is essential for deciphering its function in various cellular processes, including tissue growth, regeneration, and tumorigenesis.
The Phospho-YAP1 (S127) Polyclonal Antibody (CABP0489) is raised in rabbits and has been validated for use in Western blotting and immunofluorescence applications. Its high affinity and specificity for phosphorylated YAP1 make it a reliable tool for studying YAP1 signaling dynamics in different cell types and experimental conditions. Researchers in the fields of cancer biology, stem cell research, and developmental biology will find this antibody indispensable for their investigations into YAP1-mediated signaling pathways.
Antibody Name:
Anti-Phospho-YAP1-S127 Antibody
Antibody SKU:
CABP0489
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IHC IF
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
A synthetic phosphorylated peptide around S127 of human YAP1 (NP_001123617.1).
A synthetic phosphorylated peptide around S127 of human YAP1 (NP_001123617.1).
Purification Method:
Affinity purification
Storage Buffer:
Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:
IgG
Sequence:
AHSS P
Gene ID:
10413
Uniprot:
P46937
Cellular Location:
Cytoplasm, Nucleus
Calculated MW:
36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa
Observed MW:
68kDa
Western blot analysis of extracts of NIH3T3†’†’€š cells, using Phospho-YAP1-S127 antibody (CABP0489) at 1:1000 dilution.NIH3T3 cells were treated by Sorbitol (0.2M) at 37'C for 60 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-YAP1-S127 Rabbit pAb (CABP0489) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using Phospho-YAP1-S127 Rabbit pAb (CABP0489) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using Phospho-YAP1-S127 Rabbit pAb (CABP0489) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Synonyms:
COB1, YAP, YAP2, YAP65, YKI, YAP1
Background:
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
