The Ku80 Rabbit Monoclonal Antibody is a cutting-edge tool for researchers studying Ku80, a key protein involved in DNA repair and maintenance of genomic stability. This monoclonal antibody is specifically designed for use in various applications including Western blotting, immunofluorescence, and immunoprecipitation.Ku80 is a critical component of the DNA-dependent protein kinase complex, which plays a crucial role in repairing double-stranded DNA breaks. Dysregulation of Ku80 has been implicated in various diseases, including cancer and autoimmune disorders.
By targeting Ku80 with this high-quality monoclonal antibody, researchers can gain valuable insights into the molecular mechanisms underlying these conditions.This Ku80 Rabbit Monoclonal Antibody offers high specificity and sensitivity, ensuring accurate detection and analysis of Ku80 protein levels in different cell types and experimental settings. With its versatility and reliability, this antibody is an indispensable tool for advancing research in DNA repair, cancer biology, and other fields related to genomic stability.
Product Name:
Ku80 Rabbit Monoclonal Antibody
SKU:
CAB12338
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 633-732 of human Ku80 (P13010).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
KU80; KUB2; Ku86; NFIV; KARP1; KARP-1; Ku80
Positive Sample:
HeLa,A-549,MCF7
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus, nucleolus.
Calculated MW:
83kDa
Observed MW:
86kDa
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Purification Method:
Affinity purification
Gene ID:
7520
Clone Number:
ARC0706
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Ku80 Rabbit mAb (CAB12338) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
