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Phospho-WWOX (Tyr33) Antibody (PACO24400)

SKU:
PACO24400
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
IHC
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • Phospho-WWOX Tyr33 Antibody PACO24400
  • Phospho-WWOX Tyr33 Antibody PACO24400
€549
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Description

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Phospho-WWOX (Tyr33) Antibody (PACO24400)

The Phospho-WWOX (Tyr33) Antibody (PAC024400) is a valuable tool for researchers studying the phosphorylation of the WWOX protein, a tumor suppressor and regulator of cell death. This antibody, generated in rabbits, specifically binds to the phosphorylated form of WWOX at tyrosine 33, allowing for detection and analysis in various experimental settings.WWOX, also known as WW domain-containing oxidoreductase, plays a crucial role in mediating cell death pathways and suppressing tumor growth. Phosphorylation at tyrosine 33 is a key post-translational modification that influences WWOX function and cellular signaling processes. Research into the phosphorylation status of WWOX can provide valuable insights into its role in cancer development and progression.

The Phospho-WWOX (Tyr33) Antibody is suitable for use in Western blot applications, enabling researchers to assess the phosphorylation state of WWOX in cell lysates and tissue samples. Its high specificity and sensitivity make it a reliable tool for investigating the regulatory mechanisms of WWOX and its potential as a therapeutic target in cancer treatment. Researchers in the fields of oncology, cell biology, and molecular biology will find this antibody indispensable for their studies on WWOX signaling pathways and cancer biology.

Antibody Name:Phospho-WWOX (Tyr33) Antibody (PACO24400)
Antibody SKU:PACO24400
Size:100ul
Host Species:Rabbit
Tested Applications:ELISA, WB, IHC
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:100
Species Reactivity:Human, Mouse
Immunogen:Peptide sequence around phosphorylation site of tyrosine 33 (W-V-Y(p)-Y-A) derived from Human WWOX.
Form:Liquid
Storage Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern blot analysis of extracts from HepG2 cells treated with PMA using WWOX (Phospho-Tyr33) Antibody.The lane on the right is treated with the antigen-specific peptide.
Product ImageImmunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using WWOX (Phospho-Tyr33) antibody (left)or the same antibody preincubated with blocking peptide (right).
Background:

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). The highest normal expression of this gene is detected in hormonally regulated tissues such as testis, ovary, and prostate. This expression pattern and the presence of an SRD domain suggest a role for this gene in steroid metabolism.

Synonyms:FOR; WOX1; Aberrant WW domain-containing oxidoreductase;
UniProt Protein Function:WWOX: an enzyme which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). Expressed at high levels in hormonally regulated tissues such as testis, ovary, and prostate. Its SRD domain suggest a role for this gene in steroid metabolism. Shown to be an essential mediator of tumor necrosis factor-alpha-induced apoptosis in the mouse. May play a similar role in the human. May function as a suppressor of tumor growth. Alternative splicing of this gene generates 7 isoforms.
UniProt Protein Details:

Protein type:EC 1.1.1.-; Apoptosis; Oxidoreductase; Tumor suppressor

Chromosomal Location of Human Ortholog: 16q23

Cellular Component: cytoplasm; cytosol; Golgi apparatus; microvillus; mitochondrion; nucleus; plasma membrane

Molecular Function:coenzyme binding; cofactor binding; enzyme binding; oxidoreductase activity; protein binding; protein dimerization activity

Biological Process: negative regulation of Wnt receptor signaling pathway; osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; skeletal morphogenesis; steroid metabolic process; Wnt receptor signaling pathway

Disease: Epileptic Encephalopathy, Early Infantile, 28

NCBI Summary:This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) protein family. This gene spans the FRA16D common chromosomal fragile site and appears to function as a tumor suppressor gene. Expression of the encoded protein is able to induce apoptosis, while defects in this gene are associated with multiple types of cancer. Disruption of this gene is also associated with autosomal recessive spinocerebellar ataxia 12. Disruption of a similar gene in mouse results in impaired steroidogenesis, additionally suggesting a metabolic function for the protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Code:Q96KM3
NCBI GenInfo Identifier:635172870
NCBI Gene ID:51741
NCBI Accession:NP_001278926.1
UniProt Secondary Accession:Q96KM3,Q5MYT5, Q96KM3, Q96RF2, Q9BTT8, Q9NPC9, Q9NRF4 Q9NRF5, Q9NRF6, Q9NRK1, Q9NZC5, A8K323,
UniProt Related Accession:Q9NZC7
Molecular Weight:23,868 Da
NCBI Full Name:WW domain-containing oxidoreductase isoform 4
NCBI Synonym Full Names:WW domain containing oxidoreductase
NCBI Official Symbol:WWOX  
NCBI Official Synonym Symbols:FOR; WOX1; EIEE28; FRA16D; SCAR12; HHCMA56; PRO0128; SDR41C1; D16S432E  
NCBI Protein Information:WW domain-containing oxidoreductase
UniProt Protein Name:WW domain-containing oxidoreductase
UniProt Synonym Protein Names:Fragile site FRA16D oxidoreductase; Short chain dehydrogenase/reductase family 41C member 1
Protein Family:WW domain-containing oxidoreductase
UniProt Gene Name:WWOX  
UniProt Entry Name:WWOX_HUMAN
AntibodiesELISA Kits
Phospho-WWOX (Y33) Antibody (PACO05983)WWOX (Phospho-Tyr33) Colorimetric Cell-Based ELISA Kit
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