The Phospho-Vim (S83) Rabbit Polyclonal Antibody (CABP1119) is specifically designed for researchers studying phosphorylation of vimentin protein at serine 83. Vimentin is a key intermediate filament protein involved in maintaining cell shape and structure. This antibody, raised in rabbits, is highly sensitive and specific for detecting phosphorylated vimentin at serine 83 in human samples.Phosphorylation of vimentin at serine 83 has been linked to various cellular processes, including cell migration, invasion, and signaling pathways. This antibody is validated for use in Western blot applications, enabling researchers to study the role of phosphorylated vimentin in different cell types and biological contexts.
Understanding the phosphorylation status of vimentin at serine 83 is essential for unraveling its functions and implications in diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases. The Phospho-Vim (S83) Rabbit Polyclonal Antibody (CABP1119) provides a valuable tool for researchers investigating the regulatory mechanisms and functional consequences of vimentin phosphorylation, advancing our knowledge of cellular dynamics and disease progression.
Product Name:
Phospho-Vimentin-S83 Rabbit Polyclonal Antibody
SKU:
CABP1119
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S83 of human VIM (NP_003371.2).
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
Purification Method:
Affinity purification
Gene ID:
7431
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Vimentin-S83 antibody (CABP1119) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
