The Phospho-Vim (S83) Polyclonal Antibody (CABP0799) is a valuable tool for researchers studying vimentin, an intermediate filament protein that plays a key role in cell structure and motility. This antibody, raised in rabbits, specifically targets the phosphorylated form of vimentin at serine 83, making it ideal for investigating signaling pathways and cellular processes that involve vimentin phosphorylation.Vimentin is a versatile protein that is involved in a variety of cellular functions, including cell migration, adhesion, and signaling. Phosphorylation of vimentin at serine 83 has been linked to various biological processes, such as cell division, cell cycle regulation, and cytoskeletal rearrangements.
By using this antibody in Western blot applications, researchers can gain insights into the role of vimentin phosphorylation in different cellular contexts.Overall, the Phospho-Vim (S83) Polyclonal Antibody (CABP0799) is a reliable reagent for studies on vimentin phosphorylation and its functional implications in cell biology, cancer research, and other fields. Its high specificity and sensitivity make it a valuable tool for deciphering the intricate signaling networks that involve vimentin and its post-translational modifications.
Product Name:
Phospho-Vimentin-S83 Rabbit Polyclonal Antibody
SKU:
CABP0799
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S83 of human VIM (NP_003371.2).
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
Purification Method:
Affinity purification
Gene ID:
7431
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S83 pAb (CABP0799) at 1:2000 dilution or Vimentin antibody (CAB2584).HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
