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Phospho-Vimentin-S83 Rabbit Polyclonal Antibody (CABP0799)

SKU:
CABP0799
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Vimentin-S83 Rabbit Polyclonal Antibody
SKU:CABP0799
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:A synthetic phosphorylated peptide around S83 of human VIM (NP_003371.2).
Sequence:QDSV D
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:CTRCT30; HEL113; Vimentin; VIM; vimentin; Phospho-Vimentin-S83
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:54kDa
Observed MW:60kDa

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.

Purification Method:Affinity purification
Gene ID:7431
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S83 pAb (CABP0799) at 1:2000 dilution or Vimentin antibody (CAB2584).HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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