The Phospho-VIM (S56) Polyclonal Antibody (CABP0804) is specifically designed for research involving phosphorylated Vimentin at residue S56, a key post-translational modification that plays a role in cell migration, invasion, and cytoskeletal dynamics. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blot and immunofluorescence.Vimentin, a type III intermediate filament protein, is an essential component of the cytoskeleton and is involved in maintaining cellular structure and functionality. Phosphorylation of Vimentin at S56 is known to regulate its functions and is implicated in processes such as cell motility and cancer metastasis.
The Phospho-VIM (S56) Polyclonal Antibody enables specific detection and quantification of phosphorylated Vimentin at S56, allowing researchers to study the role of this post-translational modification in different cellular contexts. Its versatility and sensitivity make it an invaluable tool for studies in cell biology, cancer research, and other fields where understanding Vimentin phosphorylation is crucial for advancing knowledge and developing potential therapeutic strategies.
Product Name:
Phospho-Vimentin-S56 Rabbit Polyclonal Antibody
SKU:
CABP0804
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S56 of human VIM (NP_003371.2).
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
Purification Method:
Affinity purification
Gene ID:
7431
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S56 pAb (CABP0804) at 1:3000 dilution or Vimentin antibody (CAB11952).HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.HeLa cells were treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
