Anti-Phospho-VIM-S39 pAb Antibody (CABP0806)
- SKU:
- CABP0806
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
Anti-Phospho-VIM-S39 pAb Antibody (CABP0806)
The Phospho-VIM (S39) Polyclonal Antibody (CABP0806) is a valuable tool for researchers studying Vimentin, a type III intermediate filament protein involved in cell structure and motility. This antibody, raised in rabbits, is highly specific to phosphorylated Vimentin at serine 39 and is validated for use in various applications including Western blot and immunofluorescence.Vimentin is a key regulator of cellular processes such as cell adhesion, migration, and signaling, making it a crucial player in cancer progression, metastasis, and other cellular activities. Phosphorylation at serine 39 has been linked to changes in Vimentin function and cellular processes, making this antibody an essential tool for investigating the role of phosphorylated Vimentin in cancer biology and other diseases.
The Phospho-VIM (S39) Polyclonal Antibody is ideal for researchers interested in studying the phosphorylation status of Vimentin and its implications in various cellular processes. Its high specificity and reliability make it a valuable resource for studies in cell biology, cancer research, and other fields where Vimentin phosphorylation is of interest.
| Antibody Name: | Anti-Phospho-VIM-S39 Antibody |
| Antibody SKU: | CABP0806 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S39 of human VIM (NP_003371.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, NIH/3T3 |
| Immunogen: | A synthetic phosphorylated peptide around S39 of human VIM (NP_003371.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TYSL G |
| Gene ID: | 7431 |
| Uniprot: | P08670 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 53kDa |
| Observed MW: | 57KDa |
 | Western blot analysis of extracts of NIH/3T3 cells, using Phospho-VIM-S39 antibody (CABP0806) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37'C for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00071). Exposure time: 1s. |
 | Western blot analysis of extracts of HeLa cells, using Phospho-VIM-S39 antibody (CABP0806) at 1:1000 dilution.HeLa cells were treated by Paclitaxel (100 nM/ml) at 37'C for 20 hours.HeLa cells were treated by Hydroxyurea (4 mM) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded human appendix using Phospho-VIM-S39 antibody (CABP0806) at dilution of 1:100 (40x lens). |
| Synonyms: | CTRCT30, HEL113, Vimentin, VIM, vimentin |
| Background: | This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract. |
| UniProt Protein Function: | Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10p13 Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol Molecular Function:protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding Disease: Cataract 30 |
| NCBI Summary: | This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017] |
| UniProt Code: | P08670 |
| NCBI GenInfo Identifier: | 55977767 |
| NCBI Gene ID: | 7431 |
| NCBI Accession: | P08670.4 |
| UniProt Related Accession: | P08670 |
| Molecular Weight: | |
| NCBI Full Name: | Vimentin |
| NCBI Synonym Full Names: | vimentin |
| NCBI Official Symbol: | VIMÂ Â |
| NCBI Protein Information: | vimentin |
| UniProt Protein Name: | Vimentin |
| Protein Family: | Vimentin |
| UniProt Gene Name: | VIMÂ Â |
| UniProt Entry Name: | VIME_HUMAN |
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