The Phospho-VASP (S157) Monoclonal Antibody (CABP1511) is specifically designed for research involving VASP, a crucial protein involved in the regulation of actin dynamics and cell migration. This antibody, produced using hybridoma technology, exhibits high specificity and sensitivity towards phosphorylated VASP at serine 157. It is validated for use in various applications, including Western blot, immunofluorescence, and immunohistochemistry.VASP, also known as vasodilator-stimulated phosphoprotein, plays a key role in cell motility, adhesion, and cytoskeletal organization. Phosphorylation of VASP at serine 157 is a critical post-translational modification that regulates its functions in cell signaling pathways.
Understanding the regulatory mechanisms of VASP phosphorylation is essential for studying cellular processes such as cell migration, invasion, and metastasis in diseases like cancer.This Phospho-VASP (S157) Monoclonal Antibody offers researchers a reliable tool for studying the phosphorylation status of VASP in different cell types and experimental conditions. Its high specificity and sensitivity make it an essential reagent for investigating the role of VASP in physiological and pathological processes, providing valuable insights into cell biology, cancer biology, and related fields of research.
Product Name:
Phospho-VASP-S157 Monoclonal Antibody
SKU:
CABP1511
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S157 of human VASP(NP_003361.1).
Sequence:
RRVS NA
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:1000 - 1:5000
Synonyms:
VASP; Phospho-VASP-S157
Positive Sample:
HEL,HEL+Forskolin
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, cytoskeleton
Calculated MW:
40kDa
Observed MW:
50kDa
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Purification Method:
Affinity purification
Gene ID:
7408
Clone Number:
ARC64201
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HEL cells, using Phospho-VASP-S157 Rabbit mAb (CABP1511) at 1:3000 dilution.HEL cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
