The Phospho-VASP S157 Monoclonal Antibody (CABP1510) is a powerful tool for researchers studying VASP, a protein involved in cell migration and adhesion. This antibody, developed for use in various applications, including Western blot and immunofluorescence, specifically detects the phosphorylated form of VASP at serine 157. By targeting this specific phosphorylation site, researchers can gain insights into the regulation of VASP activity and its role in cellular processes. The antibody, produced using high-quality monoclonal antibodies, provides reliable and consistent results when used in experiments with human samples.
Understanding the phosphorylation status of VASP at serine 157 is crucial for unraveling its functions in cell signaling pathways and its implications in diseases such as cancer and cardiovascular disorders. The Phospho-VASP S157 Monoclonal Antibody (CABP1510) offers a valuable tool for investigating the intricate mechanisms underlying VASP biology and its potential as a therapeutic target.
Product Name:
Phospho-VASP-S157 Monoclonal Antibody
SKU:
CABP1510
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S157 of human VASP(NP_003361.1).
Sequence:
RRVS NA
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:2000 - 1:5000
Synonyms:
VASP; Phospho-VASP-S157
Positive Sample:
NIH/3T3+Forskolin,C6+Forskolin
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, cytoskeleton
Calculated MW:
40kDa
Observed MW:
50kDa
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Purification Method:
Affinity purification
Gene ID:
7408
Clone Number:
ARC64203
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Phospho-VASP-S157 Rabbit mAb (CABP1510) at 1:4000 dilution.C6 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 45s.
