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Phospho-VASP-S157 Monoclonal Antibody (CABP1510)

SKU:
CABP1510
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €609
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-VASP-S157 Monoclonal Antibody
SKU:CABP1510
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S157 of human VASP(NP_003361.1).
Sequence:RRVS NA
Tested Applications:WB ELISA
Recommended Dilution:WB,1:2000 - 1:5000
Synonyms:VASP; Phospho-VASP-S157
Positive Sample:NIH/3T3+Forskolin,C6+Forskolin
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, cytoskeleton
Calculated MW:40kDa
Observed MW:50kDa

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Purification Method:Affinity purification
Gene ID:7408
Clone Number:ARC64203
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from C6 cells, using Phospho-VASP-S157 Rabbit mAb (CABP1510) at 1:4000 dilution.C6 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 45s.

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