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Phospho-ULK1-S757 Rabbit Polyclonal Antibody (CABP0736)

SKU:
CABP0736
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Autophagy
€419
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Description

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Product Name:Phospho-ULK1-S757 Rabbit Polyclonal Antibody
SKU:CABP0736
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S757 of human Phospho-ULK1-S757 (NP_003556.1).
Sequence:TVGS P
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:ATG1; ATG1A; UNC51; hATG1; Unc51.1; Phospho-ULK1-S757
Positive Sample:A-431
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Preautophagosomal structure, cytosol.
Calculated MW:113kDa
Observed MW:145kDa

Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; positive regulation by symbiont of host autophagy; and protein phosphorylation. Located in autophagosome; cytosol; and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane; extrinsic component of omegasome membrane; and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex.

Purification Method:Affinity purification
Gene ID:8408
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from A-431 cells, using Phospho-ULK1-S757 Rabbit pAb (CABP0736) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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