The Phospho-ULK1 (S757) Polyclonal Antibody (CABP0736) is a crucial tool for studying the phosphorylation of ULK1, a key regulator of autophagy, a cellular process essential for maintaining cellular homeostasis and responding to stress. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the phosphorylated form of ULK1 at serine 757, allowing for the detection and analysis of this important post-translational modification in various cell types.ULK1 is a serine/threonine kinase that plays a vital role in initiating the formation of autophagosomes, the key structures involved in autophagy. Phosphorylation of ULK1 at serine 757 has been shown to regulate its activity and downstream signaling, making it a critical site for studying the control of autophagy in response to different stimuli.
Understanding the regulation of ULK1 phosphorylation at this site is essential for unraveling the mechanisms underlying autophagy dysfunction in various diseases, including neurodegenerative disorders, cancer, and metabolic conditions.Researchers studying autophagy, cell biology, and disease mechanisms will find the Phospho-ULK1 (S757) Polyclonal Antibody (CABP0736) to be a valuable tool for their experiments, providing insights into the regulation of autophagy and potential therapeutic targets for
Product Name:
Phospho-ULK1-S757 Rabbit Polyclonal Antibody
SKU:
CABP0736
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S757 of human Phospho-ULK1-S757 (NP_003556.1).
Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; positive regulation by symbiont of host autophagy; and protein phosphorylation. Located in autophagosome; cytosol; and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane; extrinsic component of omegasome membrane; and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex.
Purification Method:
Affinity purification
Gene ID:
8408
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-ULK1-S757 Rabbit pAb (CABP0736) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
