The Phospho-ULK1 (S555) Polyclonal Antibody (CABP0760) is specifically designed for research involving ULK1, a protein kinase involved in autophagy, a crucial cellular process for maintaining cellular homeostasis. This antibody, raised in rabbits, is highly reactive with human, mouse, and rat samples, making it versatile for use in different model systems. Validated for use in Western blot and immunohistochemistry applications, it specifically binds to the phosphorylated form of ULK1 at serine 555, allowing for precise detection and analysis in various cell types.ULK1 is a key regulator of autophagy, a process that removes damaged organelles and proteins to maintain cellular health.
Dysregulation of autophagy is implicated in various diseases, including neurodegenerative disorders, cancer, and metabolic syndromes. Understanding the phosphorylation state of ULK1, specifically at serine 555, is critical for elucidating its role in autophagy regulation and its potential as a therapeutic target. This antibody is essential for studies aiming to uncover the molecular mechanisms underlying autophagy and its implications in disease pathogenesis.
Product Name:
Phospho-ULK1-S555 Rabbit Polyclonal Antibody
SKU:
CABP0760
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S555 of human Phospho-ULK1-S555 (NP_003556.1).
Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; positive regulation by symbiont of host autophagy; and protein phosphorylation. Located in autophagosome; cytosol; and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane; extrinsic component of omegasome membrane; and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex.
Purification Method:
Affinity purification
Gene ID:
8408
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using Phospho-ULK1-S555 antibody (CABP0760) at 1:1000 dilution.293T cells were treated by Oligomycin (0.5 uM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
