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Phospho-ULK1-S555 Rabbit Polyclonal Antibody (CABP0760)

SKU:
CABP0760
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Autophagy
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-ULK1-S555 Rabbit Polyclonal Antibody
SKU:CABP0760
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S555 of human Phospho-ULK1-S555 (NP_003556.1).
Sequence:RLHS A
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:ATG1; ATG1A; UNC51; hATG1; Unc51.1; Phospho-ULK1-S555
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Preautophagosomal structure, cytosol.
Calculated MW:113kDa
Observed MW:113kDa

Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; positive regulation by symbiont of host autophagy; and protein phosphorylation. Located in autophagosome; cytosol; and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane; extrinsic component of omegasome membrane; and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex.

Purification Method:Affinity purification
Gene ID:8408
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of 293T cells, using Phospho-ULK1-S555 antibody (CABP0760) at 1:1000 dilution.293T cells were treated by Oligomycin (0.5 uM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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