null

Phospho-Ubiquitin-Ser65 Polyclonal Antibody (CABP1368)

SKU:
CABP1368
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $587
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Ubiquitin-Ser65 Polyclonal Antibody
SKU:CABP1368
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S65 of human Ubiquitin (NP_002945.1).
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:UBC; S27A; CEP80; UBA80; HEL112; UBCEP1; UBCEP80; L40; CEP52; RPL40; HUBCEP52; HEL-S-50; HMG20; Phospho-Ubiquitin-Ser65
Positive Sample:PC-3
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:18kDa/15kDa/77kDa
Observed MW:10-250kDa

Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.

Purification Method:Affinity purification
Gene ID:6233/7311/7314/7316
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from PC-3 cells, using Phospho-Ubiquitin-Ser65 Rabbit pAb (CABP1368) at 1:400 dilution.PC-3 cells were treated by Carbonyl cyanide 3-chlorophenylhydrazone(10 uM) (10 µM) at 37℃ for 6 hours after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.

Related Products