The Phospho-Ubiquitin (Ser65) Polyclonal Antibody is a valuable tool for researchers studying the role of ubiquitin phosphorylation in cellular processes. This antibody, raised in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to phosphorylated ubiquitin at serine 65, this antibody enables the detection and analysis of ubiquitin modifications in various cell types.Ubiquitin phosphorylation at serine 65 is known to have important implications in pathways related to protein degradation, DNA repair, and signal transduction.
Dysregulation of this modification has been linked to various diseases, including cancer and neurodegenerative disorders. Therefore, understanding the role of phosphorylated ubiquitin in cellular processes is essential for developing targeted therapies and interventions.Overall, the Phospho-Ubiquitin (Ser65) Polyclonal Antibody is an essential tool for researchers interested in elucidating the functions and mechanisms of ubiquitin phosphorylation in health and disease. Its versatility and specificity make it a valuable asset in molecular biology, biochemistry, and cell biology research.
Product Name:
Phospho-Ubiquitin-Ser65 Polyclonal Antibody
SKU:
CABP1368
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S65 of human Ubiquitin (NP_002945.1).
Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.
Purification Method:
Affinity purification
Gene ID:
6233/7311/7314/7316
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from PC-3 cells, using Phospho-Ubiquitin-Ser65 Rabbit pAb (CABP1368) at 1:400 dilution.PC-3 cells were treated by Carbonyl cyanide 3-chlorophenylhydrazone(10 uM) (10 µM) at 37℃ for 6 hours after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
