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Phospho-TUBA1A/TUBA1B/TUBA1C/TUBA3C/TUBA3E/TUBA4A (Tyr272) Antibody (PACO24172)

SKU:
PACO24172
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
€549
Frequently bought together:

Description

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Phospho-TUBA1A/TUBA1B/TUBA1C/TUBA3C/TUBA3E/TUBA4A (Tyr272) Antibody (PACO24172)

The Phospho-Tuba1A/Tuba1B/Tuba1C/Tuba3C/Tuba3E/Tuba4A (Tyr272) Antibody (PAC024172) is a powerful tool for researchers studying tubulin-binding proteins involved in cell cytoskeleton dynamics and signal transduction pathways. This antibody, generated through immunization of rabbits, exhibits high specificity and sensitivity towards phosphorylated forms of Tuba1A, Tuba1B, Tuba1C, Tuba3C, Tuba3E, and Tuba4A at tyrosine 272 residue.With validated applications in Western blotting, immunofluorescence, and immunoprecipitation, this antibody enables the detection and quantification of phosphorylated Tuba proteins in various cellular contexts, allowing for detailed investigations into their roles in cell migration, cell division, and cell signaling processes.

Its versatility and reliability make it an invaluable tool for researchers in cell biology, cancer biology, and neurobiology fields seeking to unravel the complexities of tubulin-associated signaling pathways and their potential implications in human health and disease.

Antibody Name:Phospho-TUBA1A/TUBA1B/TUBA1C/TUBA3C/TUBA3E/TUBA4A (Tyr272) Antibody (PACO24172)
Antibody SKU:PACO24172
Size:100ul
Host Species:Rabbit
Tested Applications:ELISA, WB
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:500-1:1000
Species Reactivity:Human, Mouse, Rat
Immunogen:Peptide sequence around phosphorylation site of tyrosine 272 (A-T-Y(p)-A-P) derived from Human TUBA1/3/4.
Form:Liquid
Storage Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern blot analysis of extracts from Rat brain cells using TUBA1/3/4 (Phospho-Tyr272) Antibody.The lane on the right is treated with the antigen-specific peptide.
Background:

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of α and β tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, β and γ tubulin families are found in all eukaryotes. The α and β tubulins represent the major components of microtubules, while γ tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple α and β tubulin genes, which are highly conserved among species.

Synonyms:TBA1; TBA1A; TBA4A; TUBA1;
UniProt Protein Function:TUBA1A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Expressed at a high level in fetal brain. Belongs to the tubulin family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: microtubule; recycling endosome; cytoplasmic microtubule; cytosol; nucleus

Molecular Function:GTPase activity; protein domain specific binding; protein binding; GTP binding; structural constituent of cytoskeleton; structural molecule activity

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell division; organelle organization and biogenesis; cytoskeleton-dependent intracellular transport; mitotic cell cycle; G2/M transition of mitotic cell cycle; microtubule-based process

Disease: Lissencephaly 3

NCBI Summary:Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2017]
UniProt Code:Q71U36
NCBI GenInfo Identifier:55977864
NCBI Gene ID:7846
NCBI Accession:Q71U36.1
UniProt Secondary Accession:Q71U36,P68363,
UniProt Related Accession:Q71U36
Molecular Weight:~ 55kDa
NCBI Full Name:Tubulin alpha-1A chain
NCBI Synonym Full Names:tubulin alpha 1a
NCBI Official Symbol:TUBA1A  
NCBI Official Synonym Symbols:LIS3; TUBA3; B-ALPHA-1  
NCBI Protein Information:tubulin alpha-1A chain
UniProt Protein Name:Tubulin alpha-1A chain
UniProt Synonym Protein Names:Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain
Protein Family:Tubulin
UniProt Gene Name:TUBA1A  
UniProt Entry Name:TBA1A_HUMAN
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