The Phospho-TSC2 (T1462) Polyclonal Antibody (CABP0866) is a valuable tool for researchers studying TSC2, a tumor suppressor protein involved in the regulation of cell growth and proliferation. This antibody, produced in rabbits, is highly specific to the phosphorylated form of TSC2 at threonine 1462 and has been validated for use in various applications, including Western blotting.TSC2 is a key player in the mTOR signaling pathway, which regulates cell growth and metabolism in response to nutrient availability and growth factors. Phosphorylation of TSC2 at threonine 1462 is known to modulate its function and impact downstream signaling cascades. By targeting this specific phospho-site, researchers can gain insights into the regulatory mechanisms of TSC2 and its role in diseases such as cancer and metabolic disorders.
This antibody offers researchers a reliable tool for studying the phosphorylation status of TSC2 in different cell types and experimental conditions. Its high sensitivity and specificity make it suitable for use in both basic research and drug development efforts aimed at targeting the mTOR pathway for therapeutic interventions. Dive into the intricate world of TSC2 signaling with the Phospho-TSC2 (T1462) Polyclonal Antibody and uncover new avenues for advancing our understanding of cell biology and disease mechanisms.
Product Name:
Phospho-TSC2-T1462 Rabbit Polyclonal Antibody
SKU:
CABP0866
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around T1462 of human TSC2 (NP_000539.2).
Sequence:
GYTI S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
LAM; TSC4; PPP1R160; Phospho-TSC2-T1462
Positive Sample:
MCF7
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Membrane, Peripheral membrane protein.
Calculated MW:
201kDa
Observed MW:
200kDa
This gene is a tumor suppressor gene that encodes the growth inhibitory protein tuberin. Tuberin interacts with hamartin to form the TSC protein complex which functions in the control of cell growth. This TSC protein complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signaling which is a major regulator of anabolic cell growth. Mutations in this gene have been associated with tuberous sclerosis and lymphangioleiomyomatosis.
Purification Method:
Affinity purification
Gene ID:
7249
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from MCF7 cells, using Phospho-TSC2-T1462 Rabbit pAb (CABP0866) at 1:1000 dilution.MCF7 cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
