The Phospho-TP53 (S315) Polyclonal Antibody (CABP0462) is a valuable tool for researchers studying the phosphorylation of the tumor suppressor protein TP53 at serine 315. This antibody, raised in rabbits, is highly specific for detecting phosphorylated TP53 in human samples and is suitable for use in Western blot applications. By targeting the phosphorylated form of TP53, researchers can investigate the role of this post-translational modification in various cellular processes, such as DNA damage response, cell cycle regulation, and apoptosis.Phosphorylation of TP53 at serine 315 is known to influence the stability and activity of the protein, impacting its function as a key regulator of cell growth and survival. Aberrant phosphorylation of TP53 has been implicated in the development and progression of cancer, making it a potential therapeutic target for cancer treatment.
By studying the phosphorylation status of TP53 at serine 315, researchers can gain insights into the molecular mechanisms underlying tumorigenesis and identify novel strategies for cancer therapy.Overall, the Phospho-TP53 (S315) Polyclonal Antibody (CABP0462) is a reliable tool for investigating the phosphorylation status of TP53 in human samples, providing valuable insights into the role of this post-translational modification in cancer and other cellular processes. Its high specificity and sensitivity make it ideal for use in research related to TP53 signaling pathways and potential
Product Name:
Phospho-p53-S315 Rabbit Polyclonal Antibody
SKU:
CABP0462
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S315 of human p53 (NP_000537.3).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-p53-S315 Rabbit pAb (CABP0462).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
