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Phospho-TIFA-T9 Polyclonal Antibody (CABP1395)

SKU:
CABP1395
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Reactivity:
Human
Isotype:
IgG
€139 - €489
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Description

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Phospho-TIFA-T9 Polyclonal Antibody (CABP1395)

The Phospho-TIFA (T9) Polyclonal Antibody (CABP1395) is a valuable tool for researchers studying TIFA, a critical signaling molecule involved in innate immune responses. This antibody, raised in rabbits, specifically detects phosphorylation at the T9 site of the TIFA protein in human samples, making it ideal for use in Western blot applications. By targeting this phosphorylation site, researchers can gain valuable insights into the regulation and activation of TIFA in various cell types.TIFA, also known as TRAF-interacting protein with forkhead-associated domain, plays a key role in the activation of NF-κB and MAPK signaling pathways in response to bacterial and viral infections.

Its involvement in innate immunity and inflammation makes it a promising target for research into infectious diseases, autoimmune disorders, and inflammatory conditions. By studying the phosphorylation of TIFA at the T9 site, researchers can uncover the mechanisms underlying its signaling functions and potential therapeutic implications.

Product Name: Phospho-TIFA-T9 Polyclonal Antibody
SKU: CABP1395
Size: 20uL, 100uL
Isotype: IgG
Host Species: Rabbit
Reactivity: Human
Immunogen: A synthetic phosphorylated peptide around T9 of human TIFA (NP_443096.1).
Sequence: ADTEE
Tested Applications: WBELISA
Recommended Dilution: WB: 1:500 - 1:1000
Synonyms: T2BP; T6BP; TIFAA; Phospho-TIFA-T9
Positive Sample: TIFA knockout (KO) 293T treated by ADP-heptose after transfected with human TIFA
Conjugate: Unconjugated
Cellular Localization: Cytoplasm
Calculated MW: 21kDa
Observed MW: 20kDa

This gene encodes an adapter protein involved in adaptive and innate immunity. This protein includes a forkhead-associated (FHA) domain that specifically binds to phosphorylated serine and threonine residues. In response to bacterial infection, the encoded host cell protein undergoes an intermolecular interaction between the FHA domain and a phosphorylated threonine that leads to protein oligomerization and stimulation of the NF-kappa B and other downstream signaling pathways. This protein exhibits reduced expression in hepatocellular carcinoma and may suppress hepatocellular carcinoma progression. This protein may also play a role in the DNA damage response.

Purification Method: Affinity purification
Gene ID: 92610
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Phospho-TIFA-T9 Polyclonal Antibody Product Image Western blot analysis of lysates from TIFA knockout (KO) 293T cells transfected with TIFA using Phospho-TIFA-T9 Rabbit pAb (CABP1395) at 1:1000 dilution incubated overnight at 4℃. TIFA knockout (KO) 293T cells transfected with TIFA were treated by ADP-heptose.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (AbGn00020).
Exposure time: 60s.

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