Anti-Phospho-TBK1-S172 pAb Antibody (CABP0847)
- SKU:
- CABP0847
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
Anti-Phospho-TBK1-S172 pAb Antibody (CABP0847)
The Phospho-TBK1 (S172) Polyclonal Antibody (CABP0847) is a valuable tool for researchers studying the TBK1 protein, a key regulator of immune and inflammatory responses. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. Its specificity for the phosphorylated form of TBK1 at serine 172 allows for precise detection and analysis in a variety of cell types, making it an essential component for studies in immunology and cancer research.TBK1 is a critical signaling molecule involved in the activation of innate immune responses, particularly in the induction of type I interferons and cytokines in response to viral infection. Dysregulation of TBK1 activity has been implicated in various diseases, including cancer, autoimmune disorders, and viral infections.
By understanding the phosphorylation status of TBK1 at serine 172, researchers can gain insights into the molecular mechanisms governing immune signaling pathways and potentially identify new therapeutic targets for immune-related disorders.Overall, the Phospho-TBK1 (S172) Polyclonal Antibody (CABP0847) offers a reliable and specific tool for investigating the role of TBK1 in immune regulation and inflammatory processes. Its application in Western blot analysis provides valuable insights into the signaling pathways controlled by TBK1, opening up new possibilities for the development of targeted therapies for immune-related diseases.
| Antibody Name: | Anti-Phospho-TBK1-S172 Antibody |
| Antibody SKU: | CABP0847 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S172 of human TBK1 (NP_037386.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | THP-1 |
| Immunogen: | A synthetic phosphorylated peptide around S172 of human TBK1 (NP_037386.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FVSL Y |
| Gene ID: | 29110 |
| Uniprot: | Q9UHD2 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 83kDa |
| Observed MW: | 83kDa |
 | Western blot analysis of extracts of THP-1 cells, using Anti-Phospho-TBK1-S172 pAb (CABP0847) at 1:1000 dilution.THP-1 cells were treated by LPS (1 ug/mL) at 37'C for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
| Synonyms: | TBK1, FTDALS4, NAK, T2K |
| Background: | The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. |
| UniProt Protein Function: | TBK1: a protein kinase of the IKK family. Can mediate NFkB activation in response to certain growth factors. Forms a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK. |
| UniProt Protein Details: | Protein type:Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Other group; IKK family Chromosomal Location of Human Ortholog: 12q14.1 Cellular Component: cytoplasm; endosome membrane; cytosol Molecular Function:protein serine/threonine kinase activity; protein binding; nucleic acid binding; phosphoprotein binding; ATP binding; protein kinase activity Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; MyD88-independent toll-like receptor signaling pathway; response to virus; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; defense response to Gram-positive bacterium; positive regulation of interferon-beta biosynthetic process; positive regulation of interferon type I production; positive regulation of interferon-beta production; toll-like receptor signaling pathway; interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; negative regulation of interferon type I production; toll-like receptor 4 signaling pathway; defense response to virus; positive regulation of interferon-alpha production Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 4 |
| NCBI Summary: | The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010] |
| UniProt Code: | Q9UHD2 |
| NCBI GenInfo Identifier: | 74761953 |
| NCBI Gene ID: | 29110 |
| NCBI Accession: | Q9UHD2.1 |
| UniProt Secondary Accession: | Q9UHD2,Q8IYV3, Q9NUJ5, A8K4S4, |
| UniProt Related Accession: | Q9UHD2 |
| Molecular Weight: | 83,642 Da |
| NCBI Full Name: | Serine/threonine-protein kinase TBK1 |
| NCBI Synonym Full Names: | TANK-binding kinase 1 |
| NCBI Official Symbol: | TBK1Â Â |
| NCBI Official Synonym Symbols: | NAK; T2KÂ Â |
| NCBI Protein Information: | serine/threonine-protein kinase TBK1; NF-kB-activating kinase; NF-kappa-B-activating kinase |
| UniProt Protein Name: | Serine/threonine-protein kinase TBK1 |
| UniProt Synonym Protein Names: | NF-kappa-B-activating kinase; T2K; TANK-binding kinase 1 |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | TBK1Â Â |
| UniProt Entry Name: | TBK1_HUMAN |
Related Products
Phospho-TBK1/NAK-S172 Monoclonal Antibody (CABP1418)
Phospho-TBK1/NAK-S172 Rabbit Monoclonal Antibody (CABP1026)
Human, Mouse and Rat Phospho-TBK1 (S172) and Total TBK1 PharmaGenie ELISA Kit (SBRS2000)
Human, Mouse and Rat Phospho-TBK1 (S172) PharmaGenie ELISA Kit (SBRS1999)
Phospho-HIST1H1B (S172) Antibody (PACO65025)
