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Anti-Phospho-TBK1-S172 pAb Antibody (CABP0847)

SKU:
CABP0847
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
€419
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Description

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Antibody Name:Anti-Phospho-TBK1-S172 Antibody
Antibody SKU:CABP0847
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around S172 of human TBK1 (NP_037386.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:THP-1
Immunogen:A synthetic phosphorylated peptide around S172 of human TBK1 (NP_037386.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FVSL Y
Gene ID:29110
Uniprot:Q9UHD2
Cellular Location:Cytoplasm
Calculated MW:83kDa
Observed MW:83kDa
Anti-Phospho-TBK1-S172 pAb (CABP0847)Western blot analysis of extracts of THP-1 cells, using Anti-Phospho-TBK1-S172 pAb (CABP0847) at 1:1000 dilution.THP-1 cells were treated by LPS (1 ug/mL) at 37'C for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Synonyms:TBK1, FTDALS4, NAK, T2K
Background:The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors.
UniProt Protein Function:TBK1: a protein kinase of the IKK family. Can mediate NFkB activation in response to certain growth factors. Forms a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK.
UniProt Protein Details:

Protein type:Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Other group; IKK family

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: cytoplasm; endosome membrane; cytosol

Molecular Function:protein serine/threonine kinase activity; protein binding; nucleic acid binding; phosphoprotein binding; ATP binding; protein kinase activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; MyD88-independent toll-like receptor signaling pathway; response to virus; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; defense response to Gram-positive bacterium; positive regulation of interferon-beta biosynthetic process; positive regulation of interferon type I production; positive regulation of interferon-beta production; toll-like receptor signaling pathway; interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; negative regulation of interferon type I production; toll-like receptor 4 signaling pathway; defense response to virus; positive regulation of interferon-alpha production

Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 4

NCBI Summary:The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010]
UniProt Code:Q9UHD2
NCBI GenInfo Identifier:74761953
NCBI Gene ID:29110
NCBI Accession:Q9UHD2.1
UniProt Secondary Accession:Q9UHD2,Q8IYV3, Q9NUJ5, A8K4S4,
UniProt Related Accession:Q9UHD2
Molecular Weight:83,642 Da
NCBI Full Name:Serine/threonine-protein kinase TBK1
NCBI Synonym Full Names:TANK-binding kinase 1
NCBI Official Symbol:TBK1  
NCBI Official Synonym Symbols:NAK; T2K  
NCBI Protein Information:serine/threonine-protein kinase TBK1; NF-kB-activating kinase; NF-kappa-B-activating kinase
UniProt Protein Name:Serine/threonine-protein kinase TBK1
UniProt Synonym Protein Names:NF-kappa-B-activating kinase; T2K; TANK-binding kinase 1
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:TBK1  
UniProt Entry Name:TBK1_HUMAN

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