The Phospho-TBK1 (Nak S172) Monoclonal Antibody is a valuable tool for researchers studying the TBK1 protein and its phosphorylation at serine 172. TBK1 is a key player in the immune response, particularly in the production of type I interferons in response to viral infection. The antibody, generated in mice, is highly specific for human samples and has been validated for use in various applications, including Western blot and immunofluorescence.Phosphorylation of TBK1 at serine 172 is important for its activation and subsequent downstream signaling pathways involved in innate immunity and antiviral responses.
This antibody enables researchers to specifically detect and study the phosphorylated form of TBK1 in different cell types, providing insights into its role in immune signaling pathways. Its utility in immunology and virology research makes it a valuable tool for investigating diseases like viral infections and autoimmune disorders.
Product Name:
Phospho-TBK1/NAK-S172 Monoclonal Antibody
SKU:
CABP1418
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A phospho synthetic peptide corresponding to residues surrounding S172 of Human TBK1/NAK.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
NAK; T2K; IIAE8; FTDALS4; Phospho-TBK1/NAK-S172
Positive Sample:
NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
84kDa
Observed MW:
84kDa
The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. The protein is also an important kinase for antiviral innate immunity response.
Purification Method:
Affinity purification
Gene ID:
29110
Clone Number:
ARC57906
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Phospho-TBK1/NAK-S172 Rabbit mAb (CABP1418) at 1:500 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
