The Phospho-TBC1D4 (T642) Polyclonal Antibody (CABP0791) is a valuable tool for researchers studying the phosphorylation of TBC1D4, a key regulator of glucose metabolism and insulin signaling. This antibody, produced in rabbits, is highly specific for detecting phosphorylation at threonine 642 of TBC1D4 in human samples, making it ideal for use in Western blot and immunohistochemistry applications.TBC1D4, also known as AS160, plays an important role in regulating glucose uptake in response to insulin signaling, making it a crucial player in metabolic disorders such as diabetes. Phosphorylation of TBC1D4 at threonine 642 is known to be important for its activity, and studying this phosphorylation event can provide insights into the mechanisms underlying insulin resistance and metabolic dysfunction.
Researchers interested in understanding the molecular mechanisms governing glucose metabolism, insulin signaling, and metabolic disorders will find the Phospho-TBC1D4 (T642) Polyclonal Antibody (CABP0791) to be a valuable tool for their studies. Its high specificity and sensitivity make it a reliable choice for detecting and analyzing phosphorylated TBC1D4 in a variety of experimental settings.
Product Name:
Phospho-TBC1D4-T642 Rabbit Polyclonal Antibody
SKU:
CABP0791
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around T642 of human TBC1D4 (NP_055647.2).
Sequence:
AHTF S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
AS160; NIDDM5; Phospho-TBC1D4-T642
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
147kDa
Observed MW:
160kDa
This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
9882
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-TBC1D4-T642 Rabbit pAb (CABP0791) at 1:2000 dilution. 293T cells were treated by IGF-1 (50ng/mL) for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
