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Phospho-TBC1D4-S588 Rabbit Polyclonal Antibody (CABP0790)

SKU:
CABP0790
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
€419
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Description

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Phospho-TBC1D4-S588 Rabbit Polyclonal Antibody (CABP0790)

Based on the information provided in the URL, the Phospho-TBC1D4 (S588) Polyclonal Antibody (CABP0790) is specifically designed for research involving the phosphorylation of TBC1D4 at serine 588. TBC1D4 is a Rab-GTPase activating protein that plays a critical role in insulin-stimulated glucose uptake in skeletal muscle. Phosphorylation at serine 588 is known to regulate TBC1D4 activity, making this antibody a valuable tool for studying the molecular mechanisms underlying insulin signaling and glucose metabolism.The antibody is raised in rabbits and has been validated for use in Western blot applications, allowing for the detection and analysis of phosphorylated TBC1D4 in various cell types.

Understanding the phosphorylation status of TBC1D4 at serine 588 is important for elucidating its role in insulin signaling pathways and its potential implications in metabolic disorders such as diabetes.Overall, the Phospho-TBC1D4 (S588) Polyclonal Antibody (CABP0790) offers researchers a reliable tool for investigating the regulatory mechanisms of TBC1D4 phosphorylation and its impact on cellular metabolism, making it an essential component for studies in the fields of diabetes research and metabolic regulation.

Product Name:Phospho-TBC1D4-S588 Rabbit Polyclonal Antibody
SKU:CABP0790
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S588 of human TBC1D4 (NP_055647.2).
Sequence:LGSV D
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:AS160; NIDDM5; Phospho-TBC1D4-S588
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:147kDa
Observed MW:160kDa

This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms.

Purification Method:Affinity purification
Gene ID:9882
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using Phospho-TBC1D4-S588 Rabbit pAb (CABP0790) at 1:2000 dilution. HeLa cells were treated by IGF-1 (50ng/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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