The Phospho-TAK1 (S439) Polyclonal Antibody (CAB9437) is a valuable tool for researchers studying TAK1, a key signaling molecule involved in the regulation of inflammatory responses and cell survival. This antibody is raised in rabbits and is highly specific to human samples, making it ideal for use in Western blot applications for the detection and analysis of phosphorylated TAK1 at serine 439.TAK1 is a crucial mediator in the activation of pro-inflammatory and anti-inflammatory pathways, making it a promising target for drug development in various diseases, including cancer, autoimmune disorders, and chronic inflammatory conditions.
Understanding the phosphorylation status of TAK1 at serine 439 is essential for unraveling the intricate signaling mechanisms that govern immune responses and cell fate decisions.By utilizing the Phospho-TAK1 (S439) Polyclonal Antibody, researchers can gain valuable insights into the role of TAK1 phosphorylation in cellular signaling pathways, paving the way for the development of novel therapeutic strategies targeting TAK1 in the treatment of inflammatory diseases and cancer.
Product Name:
Phospho-TAK1-S439 Monoclonal Antibody
SKU:
CAB9437
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic phosphorylated peptide around S439 of human TAK1 (O43318).
Sequence:
RRSI Q
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
CSCF; FMD2; TAK1; MEKK7; TGF1a; Phospho-TAK1-S439
Positive Sample:
C2C12+TGF-β
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm
Calculated MW:
67kDa
Observed MW:
75kDa
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
6885
Clone Number:
ARC2714
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using (CAB9437) at 1:1000 dilution.C2C12 cells were treated by TGF-β (10 ng/ml) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
