The Phospho-Syn1 (Ser549) Antibody (PAC024349) is a valuable tool for researchers studying the phosphorylation of Synapsin 1 at Serine 549. This antibody, produced in rabbits, demonstrates high specificity and sensitivity towards human samples, making it an ideal choice for Western blot applications. By targeting the phosphorylated Synapsin 1 protein at Ser549, this antibody enables precise detection and analysis in various cell types.Synapsin 1 is a key protein involved in regulating neurotransmitter release and synaptic vesicle trafficking in neurons. Phosphorylation of Synapsin 1 at Ser549 has been implicated in modulating synaptic transmission and plasticity, making it a crucial target for studies in neurobiology and neurodegenerative disorders.
Understanding the role of Phospho-Syn1 (Ser549) can provide valuable insights into synaptic function and potential therapeutic interventions for conditions such as Alzheimer's disease and Parkinson's disease.Overall, the Phospho-Syn1 (Ser549) Antibody is a reliable tool for researchers seeking to investigate the phosphorylation status of Synapsin 1 at Ser549 and its implications in neuronal function and synaptic plasticity.
Antibody Name:
Phospho-Syn1 (Ser549) Antibody (PACO24349)
Antibody SKU:
PACO24349
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:1000
Species Reactivity:
Human, Rat, Mouse
Immunogen:
Peptide sequence around phosphorylation site of serine 549(P-A-S(p)-P-S)derived from Rat Synapsin
Form:
Liquid
Storage Buffer:
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extract from rat brain and mouse brain tissue using Synapsin(phospho-Ser549) Antibody using.
Western blot analysis of extracts from Rat brain tissue or calf intestinal phosphatase (CIP), using Synapsin (phospho-Ser549) Antibody.
Background:
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level
Greengard, P. (1987) Mol Neurobiol 1, 81-119. Hosaka, M. et al. (1999) Neuron 24, 377-87.
Synonyms:
Syn-1; synapsin I;
UniProt Protein Function:
SYN1: neuronal phosphoprotein which associates with the cytoplasmic surface of synaptic vesicles and binds to the cytoskeleton. May function in the regulation of neurotransmitter release and of axonogenesis and synaptogenesis. Mutations may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Two differentially spiced isoforms have been reported.Protein type: VesicleChromosomal Location of Human Ortholog: Xp11.23Cellular Component: Golgi apparatus; synaptic vesicle; dendrite; cytosol; cell junctionMolecular Function: protein binding; transporter activity; actin binding; protein kinase binding; calcium-dependent protein binding; catalytic activity; ATP bindingBiological Process: synaptic transmission; metabolic process; neurotransmitter secretion; regulation of neurotransmitter secretionDisease: Epilepsy, X-linked, With Variable Learning Disabilities And Behavior Disorders
UniProt Protein Details:
NCBI Summary:
This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
