The Phospho-Syk (Y525/526) Polyclonal Antibody (CABP0542) is a valuable tool for researchers studying the Syk protein, a key player in immune signaling pathways. This antibody, raised in rabbits, is highly specific for detecting phosphorylated Syk at tyrosine residues 525 and 526 in human samples, making it an essential reagent for Western blotting and other immunological assays.Syk is a critical enzyme involved in B cell receptor signaling, T cell receptor signaling, and other immune cell activation pathways. Phosphorylation at Y525/526 is known to regulate Syk activity, leading to downstream signaling events that impact immune responses.
By targeting this phosphorylation site, researchers can gain insight into the function and regulation of Syk in health and disease.This antibody is a valuable tool for investigating the role of phospho-Syk in immune disorders, cancer, and other diseases where dysregulation of immune signaling pathways plays a significant role. Its high specificity and sensitivity make it an ideal choice for researchers looking to explore the intricate mechanisms of immune activation and regulation mediated by Syk phosphorylation.
Product Name:
Phospho-Syk-Y525/526 Rabbit Polyclonal Antibody
SKU:
CABP0542
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y525 & Y526 of human Syk (NP_003168.2).
Sequence:
ENYY KA
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
IMD82; p72-Syk; Phospho-Syk-Y525/526
Positive Sample:
Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm, cytosol.
Calculated MW:
72kDa
Observed MW:
72kDa
This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6850
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-Syk-Y525/526 Rabbit pAb (CABP0542) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
