The Phospho-Syk (Y352) /ZAP70 (Y319) Polyclonal Antibody (CABP0501) is a valuable tool for researchers studying signal transduction pathways in immune cells. This antibody is specifically designed to detect phosphorylated forms of Syk and ZAP70, two key proteins involved in the activation of immune responses.Syk and ZAP70 are tyrosine kinases that play critical roles in initiating immune cell signaling cascades in response to antigen stimulation. Phosphorylation of these proteins is essential for their activation and downstream signaling events. This antibody, raised in rabbits, is highly specific to the phosphorylated forms of Syk and ZAP70 and is validated for use in Western blot applications.
The ability to detect phosphorylated Syk and ZAP70 in various cell types opens up new possibilities for understanding immune cell activation and regulation. This antibody is an essential tool for researchers studying immune signaling pathways, inflammation, and immune-related diseases such as cancer and autoimmune disorders.Overall, the Phospho-Syk (Y352) /ZAP70 (Y319) Polyclonal Antibody (CABP0501) is a highly valuable research tool for investigating the intricate mechanisms of immune cell signaling and identifying potential therapeutic targets for immune-related diseases.
A synthetic phosphorylated peptide around Y352 of human Syk (NP_003168.2).
Sequence:
SPYA D
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
SYK/ZAP70; Phospho-Syk-Y352/ZAP70-Y319
Positive Sample:
Jurkat,Jurkat+Serum
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol, early phagosome, nucleus, plasma membrane
Calculated MW:
69kDa/72kDa/35kDa/55kDa
Observed MW:
69kDa
This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
Purification Method:
Affinity purification
Gene ID:
6850/7535
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of Jurkat cells, using Phospho-Syk-Y352/ZAP70-Y319 antibody (CABP0501) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
