The Phospho-Syk (Y323) Polyclonal Antibody (CABP0291) is a valuable tool for researchers studying signaling pathways involved in immune responses. This antibody, generated in rabbits, specifically targets the phosphorylated form of Syk at tyrosine 323, an important residue for the activation of this protein kinase.Syk is a key player in signaling cascades triggered by various immune receptors, such as B cell and T cell receptors. Phosphorylation at tyrosine 323 is known to be crucial for the activation of Syk and subsequent downstream signaling events.
The Phospho-Syk (Y323) Polyclonal Antibody allows for the detection and quantification of this activated form of Syk in various cell types and tissues.Understanding the role of Syk and its phosphorylation at specific sites like Y323 is essential for unraveling the complex mechanisms of immune cell activation and function. This antibody is validated for use in Western blot applications, providing researchers with a reliable tool for studying Syk activation in the context of immunology, cancer, and autoimmune diseases.
Product Name:
Phospho-Syk-Y323 Rabbit Polyclonal Antibody
SKU:
CABP0291
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding Y323 of human Syk
Tested Applications:
WBIF/ICC
Recommended Dilution:
WB,1:500 - 1:2000 IF/ICC,1:100 - 1:200
Synonyms:
IMD82; p72-Syk; Phospho-Syk-Y323
Positive Sample:
Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm, cytosol.
Calculated MW:
72kDa
Observed MW:
72kDa
This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6850
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells using Phospho-Syk-Y323 Rabbit pAb (CABP0291).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
