The Phospho-STK3 (T384) Rabbit Polyclonal Antibody (CABP0935) is a valuable tool for researchers studying the STK3 protein, a key player in cellular signaling pathways. This antibody, generated in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications.STK3, also known as MST2, is a serine/threonine kinase involved in various cellular processes, including cell proliferation, apoptosis, and cell migration. Phosphorylation of STK3 at threonine 384 has been linked to its activation and regulation of downstream signaling cascades.
This antibody enables the detection and analysis of phosphorylated STK3 in different cell types, making it ideal for studies in cancer research, cell biology, and signaling pathways. Understanding the role of phospho-STK3 in cellular pathways is essential for developing targeted therapies for diseases associated with dysregulated cell signaling.
Product Name:
Phospho-STK3/MST2-T384 Rabbit Polyclonal Antibody
SKU:
CABP0935
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T384 of human STK3/MST2 (NP_006272.2).
Sequence:
NATS P
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
KRS1; MST2; Phospho-STK3/MST2-T384
Positive Sample:
HeLa,NIH/3T3
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
56kDa
Observed MW:
54kDa
This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6788
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-STK3/MST2-T384 Rabbit pAb (CABP0935) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
