The Phospho-STK11 (S428) Rabbit Polyclonal Antibody (CABP1108) from AssayGenie is specifically designed for research involving the STK11 protein, also known as LKB1. This antibody is raised in rabbits and is highly reactive with human samples, making it ideal for use in Western blot applications. By binding to the phosphorylated S428 site of the STK11 protein, this antibody enables precise detection and analysis in various cell types.STK11 is a serine/threonine kinase that plays a crucial role in cell growth, metabolism, and polarity. Phosphorylation of the S428 site is known to regulate STK11 activity, making it a key target for studies in cancer research and cell signaling pathways.
Understanding the function of phosphorylated STK11 is vital for unraveling its role in tumorigenesis and metabolic disorders.By utilizing the Phospho-STK11 (S428) Rabbit Polyclonal Antibody (CABP1108), researchers can uncover insights into the molecular mechanisms that govern cell physiology and disease progression, paving the way for the development of targeted therapies in oncology and metabolic disorders.
Product Name:
Phospho-STK11-S428 Rabbit Polyclonal Antibody
SKU:
CABP1108
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S428 of human STK11 (NP_000446.1).
Sequence:
RLSA C
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
PJS; LKB1; hLKB1; Phospho-STK11-S428
Positive Sample:
NIH/3T3
Cellular Localization:
Cytoplasm, Membrane, Mitochondrion, Nucleus.
Calculated MW:
49kDa
Observed MW:
65kDa
The protein encoded by this gene is a serine/threonine kinase that regulates cell polarity and energy metabolism and functions as a tumor suppressor. Mutations in this gene have been associated with the autosomal dominant Peutz-Jeghers syndrome, as well as with skin, pancreatic, and testicular cancers.
Purification Method:
Affinity purification
Gene ID:
6794
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STK11-S428 Rabbit pAb (CABP1108) at 1:1000 dilution or LKB1 antibody (CAB2122).NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
