The Phospho-STK11 (S334) Polyclonal Antibody (CABP0601) is a valuable tool for researchers studying the phosphorylation of STK11 at serine 334, a critical event in the regulation of cellular processes such as cell proliferation and energy metabolism. This antibody, produced in rabbits, has high specificity for human samples and has been validated for use in Western blot applications.STK11, also known as LKB1, is a tumor suppressor protein that plays a key role in regulating cell growth and metabolism. Phosphorylation of STK11 at serine 334 is known to impact its functions and influence downstream signaling pathways.
This antibody allows for the detection and analysis of phospho-STK11 (S334) in various cell types, providing insights into its role in cancer, metabolic disorders, and other diseases.Researchers interested in investigating the molecular mechanisms underlying STK11 phosphorylation and its implications in disease pathogenesis will find the Phospho-STK11 (S334) Polyclonal Antibody (CABP0601) to be a valuable tool in their studies. Its high reactivity and specificity make it a reliable resource for exploring the intricate workings of cellular signaling pathways involving STK11.
Product Name:
Phospho-STK11-S334 Rabbit Polyclonal Antibody
SKU:
CABP0601
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S334 of human STK11 (NP_000446.1).
Sequence:
WRSM T
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
PJS; LKB1; hLKB1; Phospho-STK11-S334
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Membrane, Mitochondrion, Nucleus.
Calculated MW:
49kDa
Observed MW:
54kDa
The protein encoded by this gene is a serine/threonine kinase that regulates cell polarity and energy metabolism and functions as a tumor suppressor. Mutations in this gene have been associated with the autosomal dominant Peutz-Jeghers syndrome, as well as with skin, pancreatic, and testicular cancers.
Purification Method:
Affinity purification
Gene ID:
6794
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-STK11-S334 antibody (CABP0601) at 1:1000 dilution.Hela cells were treated by UV at room temperature for 30 minutes and PMA (200 nM) at 37℃ for 30 minutes after serum-starvationSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
