The Phospho-Stathmin (S16) Polyclonal Antibody (CABP0229) is a valuable tool for researchers studying the phosphorylation of Stathmin at serine 16, a critical regulatory event in microtubule dynamics and cell cycle progression. This antibody, derived from rabbit hosts, exhibits high specificity for human samples and is recommended for use in Western blot and immunohistochemistry applications.Stathmin, also known as oncoprotein 18, is a key regulator of microtubule depolymerization and plays a crucial role in cell division, migration, and signaling. Phosphorylation at serine 16 modulates Stathmin's activity, impacting its ability to promote microtubule destabilization and regulate cellular processes.
The Phospho-Stathmin (S16) Polyclonal Antibody allows for precise detection and quantification of phosphorylated Stathmin, enabling in-depth analysis of its function in various cell types and physiological contexts.Research into Stathmin phosphorylation is essential for understanding cell cycle control, cytoskeletal dynamics, and the development of cancer and other diseases characterized by aberrant proliferation. By targeting this critical phosphorylation event, the Phospho-Stathmin (S16) Polyclonal Antibody facilitates investigations into the mechanisms underlying cell growth and division, offering insights that may inform the development of novel therapeutic strategies.
Product Name:
Phospho-Stathmin 1-S16 Rabbit Polyclonal Antibody
SKU:
CABP0229
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S16 of human Stathmin 1 (NP_001138926.1).
This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3925
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa and C2C12 cells, using Phospho-Stathmin 1-S16 Rabbit pAb (CABP0229) at 1:2000 dilution. HeLa cells were treated by Nocodazole (50ng/mL) for 20 hours.C2C12 cells were treated by Nocodazole (50ng/mL) for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
