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Phospho-STAT5A-Y694 Rabbit Polyclonal Antibody (CABP0138)

SKU:
CABP0138
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€419
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Description

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Product Name:Phospho-STAT5A-Y694 Rabbit Polyclonal Antibody
SKU:CABP0138
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around Y694 of human STAT5A (NP_001275647.1).
Sequence:DGYV K
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:MGF; STAT5; Phospho-STAT5A-Y694
Positive Sample:TF-1
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:91kDa
Observed MW:90kDa

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:6776
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of TF-1 cells, using Phospho-STAT5A-Y694 antibody (CABP0138) at 1:1000 dilution.TF-1 cells were treated by G-CSF (25 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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