The Phospho-STAT5A/STAT5B (Ser725/730) Antibody (PAC024063) is a valuable tool for researchers studying the phosphorylation of STAT5A and STAT5B proteins, key regulators of cell signaling pathways involved in immune response and cell proliferation. This antibody, produced in rabbits, has high specificity and sensitivity for detecting phosphorylated forms of these transcription factors in human samples, making it ideal for use in Western blot and immunohistochemistry applications.Phosphorylation of STAT5A and STAT5B at serine residues 725 and 730 is crucial for their activation and subsequent transcriptional activity, influencing processes like cell growth, differentiation, and survival. Dysregulation of STAT5 signaling has been implicated in various diseases, including cancer and immune disorders, making this antibody a valuable tool for investigating the role of these proteins in disease pathogenesis and potential therapeutic targets.
By enabling the detection and analysis of phosphorylated STAT5A and STAT5B proteins in various cell types, the Phospho-STAT5A/STAT5B (Ser725/730) Antibody (PAC024063) provides researchers with a powerful tool for advancing research in areas such as immunology, oncology, and cell signaling. Its high specificity and reliability make it a trusted choice for studying the intricate mechanisms of STAT5-mediated signaling pathways and their contribution to disease development and progression.
Peptide sequence around phosphorylation site of serine725/730(A-P-S(p)-P-V) derived from Human STAT5A/B.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from Jurkat cells treated with EGF using Phospho-STAT5A/B (Ser725/730) antibody.The lane on the right is treated with the antigen-specific peptide.
Background:
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Ross JA, et al. (2010) J Biol Chem 285, 3582-91 Uddin S, et al. (2003) Biochem Biophys Res Commun 308, 325-30 Xue HH, et al. (2002) Int Immunol 14, 1263-71
Synonyms:
Signal transducer and activator of transcription 5B; STA5B; STAT5; transcription factor STAT5B;
UniProt Protein Function:
Carries out a dual Function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
